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Tuesday, May 5, 2020 | History

3 edition of Determinants of N4 virion RNA polymerase-promoter recognition found in the catalog.

Determinants of N4 virion RNA polymerase-promoter recognition

Maria Alexandra Glucksmann

Determinants of N4 virion RNA polymerase-promoter recognition

by Maria Alexandra Glucksmann

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Published .
Written in English


Edition Notes

Statementby Maria Alexandra Glucksmann.
Classifications
LC ClassificationsMicrofilm 90/14 (Q)
The Physical Object
FormatMicroform
Paginationxi, 180 leaves
Number of Pages180
ID Numbers
Open LibraryOL2018817M
LC Control Number90953845

  The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows: 1. An isolated nucleic acid comprising first, second, third, and fourth nucleotide sequences in serial array, wherein: the first nucleotide sequence is capable of hybridizing under stringent conditions to an RNA polymerase I-specific promoter from a eukaryotic ribosomal RNA gene and.   Metapneumovirus strains and their use in vaccine formulations and as vectors for expression of antigenic sequences. United States Patent Abstract: The present invention provides an isolated mammalian negative strand RNA virus, metapneumovirus (MPV), within the sub-family Pneumoviridae, of the family Paramyxoviridae.

The T5-like siphoviruses DT57C and DT/2, isolated from horse feces, are very closely related to each other, and most of their structural proteins are also nearly identical to T5 phage. Their LTFs (L-shaped tail fibers), however, are composed of two proteins, LtfA and LtfB, instead of the single Ltf of bacteriophage T5. In silico and mutant analysis suggests a possible branched structure of Cited by: The TATA box (also named the Goldberg-Hogness box after its discoverers) was the fi rst core promoter element identi fi ed in eukaryotic protein-coding genes. The discovery of the TATA box in emerged from a comparison of the 5 fl anking sequences in a number of Drosophila, mammalian, and viral protein- coding genes (17, 18).In virtually every RNA polymerase II-transcribed gene examined.

  Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages. DOEpatents. Studier, F. William; Dubendorff, John W. This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is .   A bacteriophage T 7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. PubMed Central. Tabor, S; Richardson, C C. The RNA polymerase gene of bacteriophage T 7 has been cloned into the plasmid pBR under the inducible.


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Determinants of N4 virion RNA polymerase-promoter recognition by Maria Alexandra Glucksmann Download PDF EPUB FB2

Unlike other bacteriophages, N4 does not require the host RNA polymerase (RNAP) for the expression of its early genes. A phage-coded, virion-encapsidated RNA polymerase (N4 vRNAP) is injected into the host cell along with the phage genome to catalyze early transcription (Falco et al.).N4 vRNAP is a sequence-specific, single-stranded DNA-binding by: Sequence and DNA structural determinants of N4 virion RNA polymerase–promoter recognition Xing Dai1,2 and Lucia B.

Rothman-Denes2,3 Departments of 1Biochemistry and Molecular Biology and 2Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois USA Coliphage N4-coded, virion-encapsidated RNA polymerase (vRNAP) is able to bind to and transcribe.

Unlike other bacteriophages, N4 does not require the host RNA polymerase (RNAP) for the expression of its early genes. A phage-coded, virion-encapsidated RNA polymerase (N4 vRNAP) is injected into the host cell along with the phage genome to catalyze early transcription (Falco et al.).N4 vRNAP is a sequence-specific, single-stranded DNA-binding protein.

Coliphage N4 virion-encapsulated, DNA-dependent RNA polymerase displays unique in vitro template requirements: all determinants of promoter recognition are present on the template strand where the enzyme recognizes specific sequences between −18 and +1, including a DNA hairpin centered at −12 (Glucksmann et al.

How is this structure Cited by: These results indicate that two types of determinants are required for promoter recognition by the N4 virion RNA polymerase: specific DNAsequences(which must interact with residues on the protein) and a stem-loop structure (determined by the presence of the upstream inverted repeats and required for productive RNA polymerase binding).Cited by: Structural Basis for DNA-Hairpin Promoter Recognition by the Bacteriophage N4 Virion RNA Polymerase Article in Molecular cell 32(5) January with 35 Reads How we measure 'reads'.

In genetics, a promoter is a sequence of DNA to which proteins bind that initiate transcription of a single RNA from the DNA downstream of it. This RNA may encode a protein, or can have a function in and of itself, such as tRNA, mRNA, or ers are located near the transcription start sites of genes, upstream on the DNA (towards the 5' region of the sense strand).

The single subunit DNA-dependent RNA polymerases (RNAPs) that are encoded by bacteriophage T7 and its relatives (e.g., T3, SP6, K11) are highly specific for their individual promoter sequences (for review, see ref.

3).Although each promoter consensus sequence is related to a common sequence that extends from −17 to +6, significant differences in the interval from −8 to −11 suggest Cited by: RNA polymerase (ribonucleic acid polymerase), abbreviated RNAP or RNApol, officially DNA-directed RNA polymerase, is an enzyme that synthesizes RNA from a DNA template.

RNAP locally opens the double-stranded DNA (usually about four turns of the double helix) so that one strand of the exposed nucleotides can be used as a template for the synthesis of RNA, a process called : BRENDA entry.

Start studying CH 8: Molecular Biology of Transcription and RNA PROCESSING. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Lucia B Rothman-Denes. University determinants of N4 virion RNA polymerase-promoter recognition.

in the template strand are required for N4 viron RNA polymerase promoter recognition. Coliphage N4 virion-encapsidated, DNA-dependent RNA polymerase (vRNAP) is inactive on double-stranded N4 DNA; however, denatured promoter-containing templates are accurately transcribed. We report that all determinants of vRNAP promoter recognition exist in the template strand, indicating that this enzyme is a site-specific, single-stranded DNA.

A GTF is a protein that is always required for a RNA polymerase to bind to its promoter (the TATA box is part of the promoter) and initiate RNA synthesis. Explain the importance of bacterial call sigma factor and its structural relationship to RNA polymerase. Abstract. The RNA polymerase (RPase) holoenzyme of Escherichia coli is composed of a core enzyme with the subunit structure α 2 ββ′, combined with one of the multiple species of σ subunits, which provides the recognition activity for two hexanucleotide sequences of promoters, generally located near and positions relative to the transcription start by: Initiation and the promoter • RNA Polymerase & Promoter binding • Startpoint, hexamer ~10 bases upstream ( box) • Heptamer ~35 bases upstream ( box) • consensus sequences box consensus sequence T 80 A 95 T 45 A 60 A 50 T ADVERTISEMENTS: Let us make an in-depth study of the synthesis of RNA: Learn about- 1.

Process of RNA Synthesis 2. Transcription in Prokaryotes and 3. Transcription in Eukaryotes. Genes are expressed by transfer of genetic information from DNA to RNA. From RNA the information is used for synthesizing proteins. RNA is synthesized from a portion [ ].

The in vitro synthesis of proteins in cell-free extracts is an important tool for molecular biologists and has a variety of applications, including the rapid identification of gene products (e.g., proteomics), localization of mutations through synthesis of truncated gene products, protein folding studies, and incorporation of modified or unnatural amino acids for functional studies.

CRYSTAL STRUCTURE OF A T7 RNA POLYMERASE-T7 PROMOTER COMPLEX. Submitted by: 3DPX Category. Proteins, Macromolecules and Viruses. Keyword(s) T7 RNA POLYMERASE. TRANSFERASE-DNA complex Digital Object Identifier (DOI) / Structural basis for initiation of transcription from an RNA polymerase-promoter complex.

Citation. This cloned high-purity Ambion T7 RNA Polymerase has a high specificity for its promoter. The enzyme will transcribe large amounts of RNA from DNA sequences downstream of the promoter and show no cross talk between promoters.

One tube contain U at a concentration of U/ L is provided. Facilitate binding of RNA polymerase to the promoter through o The from BIO at Purchase College. The RNA polymerase-promoter complex is partially digested by DNase I. The DNA is then labeled on one strand. The fragments are then broken using the Maxam-Gilbert sequencing reagents.

A control is run which is treated identically except it consists of the same .1,1H NMR of valine tRNA modified bases. Evidence for multiple conformations.,"Methyl and methylene protons of dihydrouridine 17 (hU), 6-methyladenosine 37 (M6A), 7-methylguan.Read "Sequence specificity in transcription and translation, Journal of Cellular Biochemistry" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.